Objective-To evaluate effects of transplantation of bone marrow stromal cells (BMSCs) into the CSF for the treatment of chronic spinal cord injury in dogs that had not responded by 1 month after decompressive surgery. Animals-23 dogs. Procedures-Dogs with paraplegia and loss of nociception in the pelvic limbs for at least 1 month after decompressive surgery were assigned to transplantation or control groups. Dogs in the transplantation group received BMSCs injected into the CSF 1 to 3 months after decompressive surgery. Dogs in the control group did not receive additional treatments.
Evaluation of transplantation of autologous bone marrow stromal cells into the cerebrospinal fluid for treatment of chronic spinal cord injury in dogs.
Effect of the use of carprofen in dogs undergoing intense rehabilitation after lateral fabellar suture stabilization.
Objective-To determine whether carprofen, a commercially available NSAID, would decrease perceived exertion and signs of pain in dogs and therefore increase muscle mass and hind limb function without decreasing range of motion after lateral fabellar suture stabilization. Design-Randomized, blinded, controlled clinical trial. Animals-35 dogs with cranial cruciate ligament rupture and lateral fabellar suture stabilization followed by rehabilitation. Procedures-All dogs underwent surgical stabilization of cranial cruciate ligament rupture by placement of a lateral fabellar suture.
Accuracy of pressure plate kinetic asymmetry indices and their correlation with visual gait assessment scores in lame and nonlame dogs.
Objective-To determine the accuracy of pressure plate kinetic asymmetry indices (ASIs) for diagnosis of unilateral hind limb lameness in dogs and their correlation with visual gait assessment (VGA) scores. Animals-9 healthy dogs and 16 dogs with previously diagnosed unilateral rupture of the cranial cruciate ligament and concurrent unilateral hind limb lameness. Procedures-Dogs were walked over a pressure plate to determine paw contact area (PCA), peak vertical pressure (PVP), peak vertical force (PVF), and vertical impulse (VI) of both hind limbs.
Effect of cold compression therapy on postoperative pain, swelling, range of motion, and lameness after tibial plateau leveling osteotomy in dogs.
To evaluate the effect of cold compression therapy (CCT) on postoperative pain, lameness, range of motion of the stifle joint, and swelling following tibial plateau leveling osteotomy (TPLO) in dogs.
Randomized, blinded, placebo-controlled clinical trial.
34 client-owned dogs with unilateral deficiency of a cranial cruciate ligament undergoing TPLO.
Evaluation of the use of an autologous platelet-rich fibrin membrane to enhance tendon healing in dogs.
Objective-To examine effects of an autologous platelet-rich fibrin (PRF) membrane for enhancing healing of a defect of the patellar tendon (PT) in dogs. Animals-8 adult dogs. Procedures-Defects were created in the central third of the PT in both hind limbs of each dog. An autologous PRF membrane was implanted in 1 defect/dog, and the contralateral defect was left empty. Dogs (n = 4/time period) were euthanized at 4 and 8 weeks after surgery, and tendon healing was assessed grossly and histologically via a semiquantitative scoring system. Cross-sectional area of the PTs was also compared.
Reliability and validity of a visual analogue scale used by owners to measure chronic pain attributable to osteoarthritis in their dogs.
Objective-To assess validity and reliability for a visual analogue scale (VAS) used by owners to measure chronic pain in their osteoarthritic dogs. Sample-68, 61, and 34 owners who completed a questionnaire. Procedures-Owners answered questionnaires at 5 time points. Criterion validity of the VAS was evaluated for all dogs in the intended-to-treat population by correlating scores for the VAS with scores for the validated Helsinki Chronic Pain Index (HCPI) and a relative quality-of-life scale. Intraclass correlation was used to assess repeatability of the pain VAS at 2 baseline evaluations.
Objectives: The purpose of the study was to evaluate kinematic patterns in clinically normal Labrador and Rottweiler dogs trotting on a treadmill at a constant velocity. Methods: Ten Labrador Retrievers aged from 2.2 to 5.1 years, and 10 Rottweilers aged from two to 5.9 years were used. A three-dimensional capture system was used to perform analysis of joint kinematics. Kinematic data were collected by use of a triple-camera system. The kinematic study was performed first on the right side of the dog, and then on the left side. Data were analysed by use of a motion-analysis program.
Early kinematic outcome after treatment of cranial cruciate ligament rupture by tibial plateau levelling osteotomy in the dog.
Objectives: To establish the effect of tibial plateau levelling osteotomy (TPLO) on two selected pelvic limb kinematic variables in dogs treated for clinical cranial cruciate ligament rupture (CCLR). Methods: Thirteen dogs that had incurred CCLR and were treated by TPLO walked on a treadmill whilst kinematic data on pelvic limb paw motion were acquired using motion capture equipment. Data were subject to secondary processing using matrix analysis software and statistical analysis.
In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells.
Journal: Vet J
The objective of this study was to determine the tissue density, in vitro expansion and differentiation of canine adipose tissue-derived (ASC) and bone marrow-derived (BMSC) stromal cells. Primary (P0) and cell passages 1-6 (P1-6) cell doubling numbers (CD) and doubling times (DT) were determined in fresh cells. The P0, P3, and P6 adipogenic (CFU-Ad), osteogenic (CFU-Ob), and fibroblastic (CFU-F) colony forming unit frequencies, lineage specific mRNA levels in differentiated P3 cells and composition of P3 and P6 chondrogenic pellets were assessed in cryogenically preserved cells.
Effect of dog breed and body conformation on vertical ground reaction forces, impulses, and stance times.
Objectives: To assess whether fully normalised vertical ground reaction forces and stance times obtained at a trot depend on dog breed or body conformations. Methods: Peak vertical forces (PVF), vertical impulses (VI), stance times (ST), and ratio of forelimb impulse to total impulse (RVI) of 54 dogs of seven different breeds were normalised to body weight and body size according to the theory of dynamic similarity, and were tested for differences between breeds. Breeds were Borzoi, Bernese Mountain dog, Great Dane, Labrador Retriever, Landseer, Rhodesian Ridgeback, and Rottweiler.