Objective-To isolate and characterize mesenchymal stem cells (MSCs) from canine muscle and periosteum and compare proliferative capacities of bone marrow-, adipose tissue-, muscle-, and periosteum-derived MSCs (BMSCs, AMSCs, MMSCs, and PMSCs, respectively). Sample-7 canine cadavers. Procedures-MSCs were characterized on the basis of morphology, immunofluorescence of MSC-associated cell surface markers, and expression of pluripotency-associated transcription factors.
Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum.
Objectives: To design, manufacture and analyze custom implants with functional gradation in macrostructure for attachment of amputation prostheses. Methods: The external shape of the implant was designed by extracting geometrical data of canine cadavers from computed tomography (CT) scans to suit the bone cavity. Three generations of implant designs were developed and were optimized with the help of fit/fill and mechanical performance of implant-cadaver bone assembly using CT analysis and compression testing, respectively.
Journal: Vet J
This study investigated kinematic patterns in clinically normal German Shepherd dogs (GSDs) compared to those with hip dysplasia and with no clinical signs of lameness. Two groups of GSDs, including 10 clinically healthy dogs (G1) and 10 with hip dysplasia (G2), were trotted on a treadmill at a constant speed. Kinematic data were collected by a 3-camera system and analysed by a motion-analysis program. Flexion and extension joint angles and angular velocities were determined for the shoulder, elbow, carpal, hip, stifle, and tarsal joints.
Journal: Vet J
Diagnosis of lameness in dogs by use of artificial neural networks and ground reaction forces obtained during gait analysis.
Objective-To evaluate the accuracy of artificial neural networks (ANNs) for use in predicting subjective diagnostic scores of lameness with variables determined from ground reaction force (GRF) data. Animals-21 adult mixed-breed dogs. Procedures-The left cranial cruciate ligament of each dog was transected to induce osteoarthritis of the stifle joint as part of another study. Lameness scores were assigned and GRF data were collected 2 times before and 5 times after ligament transection. Inputs and the output for each ANN were GRF variables and a lameness score, respectively.
Safety of autologous bone marrow stromal cell transplantation in dogs with acute spinal cord injury.
Journal: Vet Surg
OBJECTIVE: To assess the feasibility and safety of transplantation of autologous bone marrow stromal cell (BMSC) in dogs with acute spinal cord injury (SCI). STUDY DESIGN: An open-label single-arm trial. ANIMALS: Dogs (n = 7) with severe SCI from T6 to L5, caused by vertebral fracture and luxation. METHODS: Decompressive and stabilization surgery was performed on dogs with severe SCI caused by vertebral fracture and luxation. Autologous BMSCs were obtained from each dog's femur, cultured, and then injected into the lesion in the acute stage.
Comparison of body weight distribution, peak vertical force, and vertical impulse as measures of hip joint pain and efficacy of total hip replacement.
Journal: Vet Surg
To determine whether there is a difference between the ability of peak vertical force (PVF), vertical impulse (VI), and percentage body weight distribution (%BW(dist) ) in differentiating which leg is most affected by hip joint pain before total hip replacement (THR) surgery, and for measuring changes in limb use after THR surgery.
Prospective clinical study.
Dogs (n = 47).
The effect of shock wave therapy on patellar ligament desmitis after tibial plateau leveling osteotomy.
Journal: Vet Surg
To determine if shock wave therapy (SWT) after tibial plateau leveling osteotomy (TPLO) has a beneficial effect on patellar ligament inflammation assessed by thickening of the ligament and ligament fiber disruption.
Prospective, randomized, controlled clinical trial.
Dogs (n = 30).
MATERIALS AND METHODS:
Osteochondrosis dissecans of the tarsus in Labrador Retrievers: Clinical signs, radiological data and force plate gait evaluation after surgical treatment.
Objectives: To report the outcome of unilateral surgery, by a plantaromedial approach, in 15 Labrador Retrievers with osteochondrosis dissecans (OCD) of the medial trochlear ridge of the talus. To evaluate the influence on outcome of age at surgery, bilateral involvement, periarticular soft tissue increase (PSI), osteophyte size (OS), decrease of range of joint motion and follow-up. To assess the extent of tarsal hyperextension in the standing dog. Methods: A retrospective clinical and radiographic study was conducted and the post-treatment gait was analysed using a force plate.
The precision and repeatability of a custom-made pointer device for determination of virtual landmarks in canine three-dimensional kinematics.
Placement of markers on anatomical landmarks represents a large source of error in three-dimensional kinematics. Our objectives were to test the accuracy and precision of a custom-made pointer and compare it to conventional skin markers in dogs. The pointer was first assessed by pointing at the surface of a spherical marker of known dimensions and position in space. Secondly, a point located cranio-distally to the lateral epicondyle was marked in 12 canine elbows with a Steinmann pin and reflective markers. Ability to locate a landmark was compared between the pointer and skin-mounted marker.