Stem cells

Authors: Shannon J. Murray, DVM, MSc; Kelly S. Santangelo, DVM; Alicia L. Bertone, DVM, PhD
Journal: American Journal of Veterinary Research

Objective—To evaluate early cellular influences of bone morphogenetic protein (BMP)12 and BMP2 on equine superficial digital flexor tenocytes (SDFTNs) and equine bone marrow–derived mesenchymal stem cells (BMDMSCs).

Animals—9 adult clinically normal horses.

Category: Equine - Stem cells - Tendon
Authors: L. C. Berg, T. G. Koch, T. Heerkens, K. Bessonov, P. D. Thomsen, D. H. Betts
Journal: Veterinary and Comparative Orthopaedics and Traumatology

Objective: Orthopaedic injury is the most common cause of lost training days or premature retirement in the equine athlete. Cell-based therapies are a potential new treatment option in musculo-skeletal diseases. Mesenchymal stromal cells (MSC) have been derived from multiple sources in the horse including bone marrow and umbilical cord blood. The objective of this study was to provide an in vitro comparison of the chondrogenic potential in MSC derived from adult bone marrow (BM-MSC) and umbilical cord blood (CB-MSC).

Category: Equine - Stem cells
Authors: MARTIN A. VIDAL, BVSc, MS, Diplomate ACVS , SANDRA O. ROBINSON, BS , MANDI J. LOPEZ, DVM, MS, PhD, Diplomate ACVS et al.
Journal: Veterinary Surgery

Objective—To compare the chondrogenic potential of adult equine mesenchymal stem cells derived from bone marrow (MSCs) or adipose tissue (ASCs). Study Design—In vitro experimental study. Animals—Adult Thoroughbred horses (n=11). Methods—BM (5 horses; mean [±SD] age, 4±1.4 years) or adipose tissue (6 horses; mean age, 3.5±1.1 years) samples were obtained. Cryopreserved MSCs and ASCs were used for pellet cultures in stromal medium (C) or induced into chondrogenesis±transforming growth factor-3 (TGFβ3) and bone morphogenic factor-6 (BMP-6).

Category: Cartilage - Equine - Stem cells
Authors: Alan J. Nixon, BVSc, MS; Linda A. Dahlgren, DVM, PhD; Jennifer L. Haupt, BS; Amy E. Yeager, DVM; Daniel L. Ward, PhD
Journal: American Journal of Veterinary Research

Objective—To assess the potential of adipose-derived nucleated cell (ADNC) fractions to improve tendon repair in horses with collagenase-induced tendinitis. Animals—8 horses. Procedures—Collagenase was used to induce tendinitis in the superficial digital flexor tendon of 1 forelimb in each horse. Four horses were treated by injection of autogenous ADNC fractions, and 4 control horses were injected with PBS solution. Healing was compared by weekly ultrasonographic evaluation. Horses were euthanatized at 6 weeks.

Category: Equine - Stem cells - Tendon